Biomedical EPR

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Format: Hardcover
Pub. Date: 2004-09-01
Publisher(s): Plenum Pub Corp
List Price: $398.99

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Summary

Biomedical EPR - Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling Motion in Biological Membranes Applications to Structure Determination in Proteins Discussion of Trends in EPR Technology and Prognosis for the Future

Author Biography

Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.

Table of Contents

Section I. Instrumentation and Methodology
Saturation Recovery EPR
Sandra S. Eaton
Gareth R. Eaton
Motivation
3(1)
Brief History
4(1)
Information Content of Saturation Recovery Curves
5(1)
Practical Aspects of Experimental Methodology
5(5)
Applications
10(5)
Prognosis
15(1)
References
15(4)
Loop-Gap Resonators
George A. Rinard
Gareth R. Eaton
Introduction
19(1)
History
20(2)
Why should one use loop-gap resonators?
22(1)
Basics
23(2)
Topologies of loop gap resonators
25(4)
Coupling to Resonators
29(2)
Design equations
31(4)
Magnetic Field Modulation
35(1)
LGR for Time Domain EPR
36(4)
Selection of the Q of a LGR
40(2)
Measuring B1 in the LGR
42(2)
Variable Temperature
44(1)
Mechanical Considerations
44(1)
Commercial Resonators
45(1)
Applications of Lumped-Circuit Resonators
45(2)
Further information
47(1)
References
47(6)
EPR Interfaced To Rapid Mixing
Charles P. Scholes
Introduction
53(2)
The Loop Gap Resonator Based Stopped-Flow System
55(7)
Dielectric Resonator-based Stopped-Flow EPR
62(17)
Applications of Stopped-Flow and Flow EPR to Naturally Occurring Transient Radicals
79(4)
Future Developments and Applications of Flow and Stopped-Flow EPR
83(1)
References
84(5)
Application of Angle-Selected Electron Nuclear Double Resonance to Characterize Structured Solvent in Small Molecules and Macromolecules
Devkumar Mustafi
Marvin W. Makinen
Introduction
89(4)
ENDOR Assignment of Molecular Structure and Conformation with VO2+ and Nitroxyl Spin-Labels
93(9)
ENDOR Characterization of Structured Solvent in Small Molecule Complexes and in Proteins
102(30)
Future Perspectives and Concluding Remarks
132(3)
References
135(10)
Solution-ENDOR of Some Biologically Interesting Radical Ions
Fabian Gerson
Georg Gescheidt
Solution ENDOR Spectroscopy
145(7)
Quinones
152(5)
Porphyrinoids
157(5)
References
162(3)
Electron-Electron Double Resonance
Lowell D. Kispert
Introduction
165(6)
Instrumental Techniques
171(9)
Dynamics of Biomolecules in Liquid Crystals, Glassy Solids, Polymers and Crystals
180(6)
Practical Aspects of Measurements
186(1)
References
187(12)
Digital Detection by Time-Locked Sampling in EPR
James S. Hyde
Theodore G. Camenisch
Joseph J. Ratke
Robert A. Strangeway
Wojciech Froncisz
Introduction
199(4)
Time Locking and Superheterodyne Detection - EPR Instrument Design Background
203(1)
Time-Locked Subsampling Detection for CW EPR
204(5)
Pulse Saturation Recovery Using Time-Locked Subsampling
209(3)
Selected Engineering Considerations
212(8)
Conclusion
220(1)
References
221(2)
Measurement of Distances Between Electron Spins Using Pulsed EPR
Sandra S. Eaton
Gareth R. Eaton
Introduction
223(1)
Fundamental Principles of Interaction between Electron Spins
224(3)
Distance between Two Slowly Relaxing Centers
227(1)
Distance between a Slowly Relaxing Center and a Rapidly-Relaxing Center
228(1)
Some Practical Considerations
229(1)
Recent Examples for Distances between Two Slowly-Relaxing Radicals
230(2)
Recent Examples for Distances between a Rapidly-Relaxing and a Slowly-Relaxing Spin
232(2)
Prognosis
234(1)
References
235(4)
Section II. Motion, Proteins, and Membranes
ESR and Molecular Dynamics
Jack H. Freed
Motional Narrowing and Organic Radicals
239(2)
Double Resonance and Molecular Dynamics
241(1)
Slow Motional ESR and Molecular Dynamics
242(4)
High Field ESR and Molecular Dynamics
246(5)
Spin-Echoes and Molecular Dynamics
251(5)
Two-Dimensional Fourier Transform ESR
256(7)
Prospectus
263(1)
Glossary of Abbreviations
264(1)
References
264(5)
SDSL: A Survey of Biological Applications
Candice S. Klug
Jimmy B. Feix
Introduction
269(2)
Solvent accessibility
271(9)
Motion
280(10)
Distance measurements
290(8)
Methodology
298(2)
Conclusion
300(1)
References
300(9)
Saturation Transfer Spectroscopy of Biological Membranes
Derek Marsh
Laszlo I. Horvath
Tibor Pali
Vsevolod A. Livshits
Introduction
309(2)
Historical Development
311(2)
Rapid-Passage Saturation-Transfer-EPR Displays
313(2)
Modulation-Coupled Bloch Equations
315(5)
Slow Rotational Diffusion
320(4)
Applications: Slow Rotation
324(7)
T1-Sensitive Nonlinear EPR Displays
331(8)
Slow Exchange and Paramagnetic Enhancements
339(9)
Applications: Relaxation Enhancements
348(10)
Outlook
358(5)
References
363(6)
Saturation Transfer EPR: Rotational Dynamics of Membrane Proteins
Albert H. Beth
Eric J. Hustedt
Introduction
369(4)
Methods for Analysis of ST-EPR Data
373(3)
Overview of Theory for Calculation of ST-EPR Spectra
376(6)
Nonlinear Least Squares Methods of Data Analysis
382(1)
Model Calculations of ST-EPR Spectra Using the Transition Rate Matrix Approach
383(13)
Applications of ST-EPR to Membrane Proteins
396(5)
References
401(8)
Trends in EPR Technology
James S. Hyde
Introduction
409(1)
Resonators
410(5)
Noise
415(5)
Multifrequency EPR
420(3)
EPR for Routine Analysis
423(2)
Discussion
425(1)
References
426
Prognosis
Sandra S. Eaton
Gareth R. Eaton
Contents of Previous Volumes
Index

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